Jubei-chan the Ninja Girl: Secret of the Lovely Eyepatch S01 E07 She Had Grasped the Secret Before She Knew It!

Jubei II defeats the assassins. Afterward, Shiro and Jubei II engage in a duel, he cuts off the Eyepatch, but as soon as Jiyu returns to her normal self, she collapses. Bantaro takes her to a doctor while Hajime attacks Shiro, and takes the Eyepatch.